By Frank Parker
This publication isn't really meant to be a simple textual content in infrared spectroscopy. Many such books exist and i've noted them within the textual content. relatively, i've got attempted to discover functions that may be attention-grabbing to numerous humans: complicated undergraduate chemistry scholars, graduate scholars and learn staff in numerous disciplines, spectros copists, and physicians lively in examine or within the perform of medication. With this target in brain there has been no purpose to have exhaustive insurance of the literature. I should still prefer to recognize my use of a number of books and studies, which have been beneficial in my look for fabric: G. H. Beaven, E. A. Johnson, H. A. Willis and R. G. 1. Miller, Molecular Spec troscopy, Heywood and corporate, Ltd., London, 1961. J. A. Schell guy and Charlotte Schellman, 'The Conformation of Polypeptide Chains in Proteins," within the Proteins, Vol. II, 2d Ed. (H. Neurath, ed.), educational Press, big apple, 1964. R. T. O'Connor, "Application of Infrared Spectrophotometry to Fatty Acid Derivatives," J. Am. Oil Chemists' Soc. 33, 1 (1956). F. L. Kauffman, "Infrared Spectroscopy of fat and Oils," J. Am. Oil Chemists' Soc. 41,4 (1964). W. J. Potts, Jr., Chemical Infrared Spectroscopy, Vol. I, thoughts, Wiley, long island, 1963. R. S. Tipson, Infrared Spectroscopy of Carbohydrates, nationwide Bureau of criteria Monograph llO, Washington, D.C., 1968. C. N. R. Rao, Chemical functions of Infrared Spectroscopy, educational Press, ny, 1963.
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Extra info for Applications of Infrared Spectroscopy in Biochemistry, Biology, and Medicine
CCI 4 (gas) Carbon tetrachloride vapor~oming from leaky cells containing carbon tetrachloride solutions has been known to lie in "pools" trapped in a spectrometer housing. CCI 4 After a carbon tetrachloride solution has been examined in a cell, incomplete flushing of the cell may leave behind some carbon tetrachloride, and there will be false bands at 787 and 763 cm - , . 1 vol %carbon tetrachloride gives an absorbance of ca. 1-mm cell. 3 (Continued) cm- 1 Compound or structural group 730 Polyethylene 727 Na 2 SiF 6 719 697 Polyethylene Polystyrene 667 CO 2 Band position, Comments Polyethylene now is so widely used for making all kinds of laboratory ware that it appears occasionally as a contaminant in samples.
0. , 1600 1500 1400 1300 1200 1100 1000 900 800 700 600 ~O Fig. 2. Spectra of Fluorolube and Nujol (capillary films). ) polysaccharides have frequently been cast as films from aqueous solutions onto silver chloride plates. To obtain mull spectra, however, one must use other methods. For example, a little finely powdered sodium chloride (particles < 211 in size) admixed with the sample in the mortar can act as an abrasive. If the material is quite hard, a fingernail file may be used to produce a powder which can be collected in the mortar.
Besic. F. C. Zimmerman. S. 0 .. and Wiemann, M. R .. Jr. J. Dental. Res. 41. 718 (\962). Brill. A. S. and Sandberg, H. E. Biophys. J. 8, 669 (\968). Chance. B. in Biochemistry o/Copper (J. Peisach. P. Aisen. and W. E. Blumberg. ). Academic Press. New York, 1966, p. 293. Crowell, E. , Kuhn, W. , Resnik, F. , and Varsel, C. J. Tobacco, New York 152, 20 (\961). , and Williams, R. J. P. Biopolymers Symp. 1,271 (1964). Day. P .. Smith, D. , and Williams. R. J. P. Biochemistry 6, 1563 (1967a). , Smith, D.